Current Grant Support

PI: Wilson, Co PI, James G. Fox

Grant Period: 7/01/15 - 6/30/20
Funded by: NIH
Title: Etiological Studies of Gastric Carcinoma (Project 2)
Description: This project tests the hypothesis that progression to gastric cancer is influenced not only by the genotype of H. pylori but also by concurrent infection with parasites which can modulate systemic immune repsonses and the Th1/Th2 gastric cytokine profile.


PI: John Essigmann

Grant Period: 4/1/2005 - 3/31/2020
Funded by: NIH via MIT Center for Environmental Health Sciences Core
Title: Animal Models and Pathology Core (Fox) Project Goals: To provide Center members with the latest technology for the application of animal models to environmental health research.
Specific Aims:

  1. To provide histopathology support and characterization of the molecular pathogenesis of mouse models used by CEHS members.
  2. To rederive transgenic and other specialized mouse strains by embryo transfer to assure specific pathogen-free status of all mice used by CEHS members.
  3. To provide colony management of mouse models for use by Center members and as needed provide experimental surgery or gnotobiotic support.
  4. To generate transgenic animals by pronuclear microinjection of DNA constructs, embryonic stem cell manipulation, or gene silencing using RNAi technology.
  5. To facilitate the translational research activities of Center members by providing expert technical assistance in developing and working with animal models of human disease.

PI: Tim Wang

Grant Period: 12/7/2016 - 11/30/2023
Funded by: NIH via Columbia University
Title: The Role of Stem Cells and the Microenvironment in Gastrointestinal Cancers
Project Goals: Investigate the role of Helicobacter as a tumor promoter. In this grant a mouse model is used to examine the mechanisms by which Helicobacter infections contribute to the malignant process.
Specific Aims:

  1. Provide ongoing histopathological support for the in vivo models to be utilized in the specific aims outlined.
  2. Where needed, provide special immunohistochemistry assays on target tissues, qPCR for tissue cytokines/chemokines, and serologic assessment of biomarkers utilizing the Luminex platform.

PI: Wilson

Grant Period: 7/1/2015 - 6/30/2020
Funded by: NIH via Columbia University
Title: Etiological Studies of Gastric Carcinoma
Project Goals: This project tests the hypothesis that progression to gastric cancer is influenced not only by the genotype of H. pylori but also by concurrent infection with parasites which can modulate systemic immune responses and the Th1/Th2 gastric cytokine profile.
Specific Aims:

  1. To characterize the gastric microbiota from patients at high and low risk for gastric cancer and evaluate the ability of specific microbiota to modulate carcinogenesis.
  2. To determine whether activity of IL-11 and IL-22 is associated with the development of gastric cancer in high risk patients and in the murine INS-GAS model.
  3. Characterize the impact of intestinal helminths and/or select bacteria identified in high-risk (HR) and low-risk (LR) patients on gastric carcinogenesis in the INS-GAS model; determine if the mechanism involves modulation of the IL22/STAT3/IL11.

PI: Wang

Grant Period: 5/3/2017 – 4/30/2022
Funded by: NIH via Columbia University
Title: The Role of the Microenvironment in Barrett's Esophagus
Project Goals: Use a germ free mouse model to determine the role of the microbiota in esophageal cancer formation.
Specific Aims:

  1. Rederive Vim-Cre into axenic health status and cross with Ins-Gas mice
  2. Induce HCC in germ-free and SPF mice by DEN and CCl4 and by orthotopic transplantation
  3. Induce HCC in germ-free and SPF mice by DEN and CCl4 and by orthotopic transplantation
  4. Isolate myofibroblasts from HCCs in Vim-Cre mice 5. Pathway analysis